Detect Range:31.25-2000 pg/mL
Sensitivity:14.5 pg/mL
Sample Type:Serum, Plasma, Other biological fluids
Sample Volume: 1-200 μL
Assay Time:1-4.5h
Detection wavelength:450 nm

Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate IL-10 in samples. An antibody specific for IL-10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIL-10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-10 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-10 bound in the initial step. The color development is stopped and the intensity of the color is measured.