Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate EFNA2 in samples. An antibody specific for EFNA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyEFNA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Ephrin-A2 is a member of the ephrin family. The protein is composed of a signal sequence, a receptor-binding region, a spacer region, and a hydrophobic region. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system.
Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. Posttranslational modifications determine whether this protein localizes to the nucleus or the cytoplasm.
