Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate E2F3 in samples. An antibody specific for E2F3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyE2F3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for E2F3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of E2F3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:E2F3 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F1 and E2F2, have an additional cyclin binding domain. This protein binds specifically to retinoblastoma protein pRB in a cell-cycle dependent manner.
