• Product NameCAPN3 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen Affinity Purified
• ApplicationsWB
• Species ReactivityHu Ms
• SpecificityThe antibody detects endogenous level of total CAPN3 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Calpain-3 protein (582-821aa)
• Target NameCAPN3
• Alternative Names    Calcium-activated neutral proteinase 3, CANP 3, Calpain L3, Calpain p94, Muscle-specific calcium-activated neutral protease 3, New calpain 1, nCL-1, CAPN3, CANP3, CANPL3, NCL1
• Accession No. Swiss-Prot#: P20807
• UniprotP20807
• Gene ID825;
• Calculated MW 94kd
• Concentration 1.0mg/mL
• Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:2000
  

Calcium-regulated non-lysosomal thiol-protease.

[1]Clinical, pathological, and genetic features of limb-girdle muscular dystrophy type 2A with new calpain 3 gene mutations in seven patients from three Japanese families."Kawai H., Akaike M., Kunishige M., Inui T., Adachi K., Kimura C., Kawajiri M., Nishida Y., Endo I., Kashiwagi S., Nishino H., Fujiwara T., Okuno S., Roudaut C., Richard I., Beckmann J.S., Miyoshi K., Matsumoto T. Muscle Nerve 21:1493-1501(1998). [2]Pseudometabolic expression and phenotypic variability of calpain deficiency in two siblings."Penisson-Besnier I., Richard I., Dubas F., Beckmann J.S., Fardeau M.Muscle Nerve 21:1078-1080(1998). [3]A small in-frame deletion within the protease domain of muscle-specific calpain, p94 causes early-onset limb-girdle muscular dystrophy 2A."Haeffner K., Speer A., Huebner C., Voit T., Oexle K.Hum. Mutat. Suppl. 1:S298-S300(1998).

• All lanes: Calpain-3 antibody at 5ug/ml+mouse brain tissue secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :94kDa observed band size :94kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting