Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DYNC1H1 in samples. An antibody specific for DYNC1H1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDYNC1H1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DYNC1H1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DYNC1H1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Dyneins are a group of microtubule-activated ATPases that serve to convert chemical energy into mechanical energy. Cytoplasmic dynein has been implicated in a variety of other forms of intracellular motility, including retrograde axonal transport, protein sorting between apical and basolateral surfaces, and redistribution of organelles like endosomes and lysosomes. The predicted partial protein sequence shares 99% and 34% identity with rat DHC1a and human DNHC2, respectively. Antibodies against DHC1 recognized a high molecular mass protein on Western blots of extracts from several mammalian cell lines. Northern blot analysis revealed that DHC1 is expressed as an approximately 15-kb mRNA in several mammalian cells lines and human tissues, including those that make neither cilia nor flagella.
