Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DRD2 in samples. An antibody specific for DRD2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDRD2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DRD2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:D2 (DRD2). There is a short version of D2 (D2Sh) and a long version of D2 (D2Lh): The D2Sh are pre-synaptic situated, having modulatory functions (called autoreceptor, they regulate the neurotransmission by feed-back mechanisms, i.e., synthesis, storage and release of dopamine into the synaptic cleft).
The D2Lh may have the classic function of a post-synaptic receptor, i.e., keep going on the neurotransmission (excitatory or inhibitory) once blocked by a receptor antagonist or stimulated by the endogenous neurotransmitter itself or a synthetic full or partial agonist.D2-like activation is coupled to the G protein Gαi, which subsequently increases phosphodiesterase activity. Phosphodiesterases break down cAMP, producing an inhibitory effect in neurons.
