Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DRD1 in samples. An antibody specific for DRD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDRD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DRD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DRD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Dopamine is an endogenous catecholamine that influences many cellular activities, including behavior, hormone synthesis and release, blood pressure and intracellular ion transport.
DRD1 gene encodes the D1 subtype of the dopamine receptor. The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
