Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DNHD1 in samples. An antibody specific for DNHD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDNHD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DNHD1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DNHD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DNHD1 Belongs to the dynein heavy chain family. Dynein was first isolated from Tetrahymena cilia four decades ago. The analysis of the primary structure of the dynein heavy chain and the discovery that many organisms express multiple dynein heavy chains have led to two insights. One, dynein, whose motor domain comprises six AAA modules and two potential mechanical levers, generates movement by a mechanism that is fundamentally different than that which underlies the motion of myosin and kinesin. And two, organisms with cilia or flagella express approximately 14 different dynein heavy chain genes, each gene encodes a distinct dynein protein isoform, and each isoform appears to be functionally specialized. Sequence comparisons demonstrate that functionally equivalent isoforms of dynein heavy chains are well conserved across species.
