• Product NameTyrosyl-tRNA synthetase, cytoplasmic Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Tyrosyl-tRNA synthetase, cytoplasmic polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Tyrosyl-tRNA synthetase, cytoplasmic protein
• Target NameTyrosyl-tRNA synthetase, cytoplasmic
• Alternative Names    YARS, Tyrosyl--tRNA ligase, TyrRS
• Accession No. Swiss-Prot#: P54577
• UniprotP54577
• Gene ID8565;
• Calculated MW 58kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA(Tyr)

[1]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. Venter J.C.[2]

• All lanes; Tyrosyl-tRNA synthetase, cytoplasmic Antibody at 2ug/ml + 293T whole cell lysate at 20 ug Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 58 kDa Observed band size :58 kDa
• Immunohistochemical analysis of paraffin-embeded human kidney using #42104 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting