Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMXL2 in samples. An antibody specific for DMXL2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMXL2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMXL2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DMXL2 shares significant homology with DMXL1. RT-PCR ELISA detected moderate expression in brain, lung, liver, kidney, testis, and ovary. Expression was low in heart and undetectable in skeletal muscle, pancreas, and spleen.
The deduced 3,036-amino acid protein has a calculated molecular mass of 339.8 kD and contains 12 WD domains. Western blot analysis of rat tissues showed abundant expression of Dmxl2 in brain only, and subcellular distribution analysis revealed enrichment in the synaptic vesicle fraction. Immunofluorescence and immunoelectron microscopy of mouse hippocampus and primary cultured rat hippocampal neurons showed that Dmxl2 was concentrated on synaptic vesicles at synapses.
