Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMXL1 in samples. An antibody specific for DMXL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMXL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMXL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMXL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By EST database searching for sequences showing homology to Drosophila DmX and by use of primers designed for RT-PCR from human placental mRNA, Kraemer et al. (2000) cloned a nearly full-length homolog, which they designated DMX-like-1. The DMXL1 cDNA encodes a deduced 3,027-amino acid protein with a calculated mass of approximately 338 kD. The protein belongs to the superfamily of WD repeat proteins, most of which have regulatory functions, and contains at least 28 WD repeat units. DMXL1 shows a high level of evolutionary conservation. By searching EST databases with the complete cDNA sequence as query, Kraemer et al. (2000) found that DMXL1 is expressed in a variety of tissues, including bone, breast, eye, foreskin, heart, parathyroid, small intestine, testis, tonsils, uterus, and whole embryo.
