Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMRTC2 in samples. An antibody specific for DMRTC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DMRTC2 belongs to the DMRT family. Contains 1 DM DNA-binding domain. Expressed in testis and pancreas. May be involved in sexual development. Recent advances in the evolutionary genetics of sex determination indicate that DMRT1 may be a vertebrate equivalent of the Drosophila melanogaster master sex regulator gene, doublesex. The role of DMRT1 seems to be confined to some aspects of male sex differentiation, whereas in Drosophila, doublesex has wider developmental effects in both sexes. This suggests other homologs of doublesex may exist in the vertebrate genome and encode sex-specific functions not displayed by DMRT1. Human DM genes map to three well-defined regions of chromosomes 1, 9, and 19 (one gene on chromosome 19 having an additional homolog on chromosome X).
