• Product NameSUMO-conjugating enzyme UBC9 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total SUMO-conjugating enzyme UBC9 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human SUMO-conjugating enzyme UBC9 protein
• Target NameSUMO-conjugating enzyme UBC9
• Alternative Names    UBE2I, UBC9, UBCE9, SUMO-protein ligase, Ubiquitin carrier protein 9, Ubiquitin carrier protein I, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, p18
• Accession No. Swiss-Prot#: P63279
• UniprotP63279
• Gene ID7329;
• Calculated MW 17.4kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Accepts the ubiquitin-like proteins SUMO1, SUMO2, SUMO3 and SUMO4 from the UBLE1A-UBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Necessary for sumoylation of FOXL2 and KAT5. Essential for nuclear architecture and chromosome segregation

[1]"Identification of the structural and functional human homolog of the yeast ubiquitin conjugating enzyme UBC9."Yasugi T., Howley P.M.Nucleic Acids Res. 24:2005-2010(1996) [2]"Assignment of the gene for a ubiquitin-conjugating enzyme (UBE2I) to human c

• All lanes : SUMO-conjugating enzyme UBC9 antibody at 2ug/ml Lane 1 : 293T whole cell lysate Lane 2 : EC109 whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 17.4kDa Observed band size: 17.4kDa
• Immunohistochemical analysis of paraffin-embeded human prostate using #42105 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting