Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMRTB1 in samples. An antibody specific for DMRTB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTB1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:In humans, the DMRT genes encode a large family of transcription factors that are related to the Drosophila doublesex proteins. Expressed primarily in the gonads, the DMRT proteins contain cysteine-rich DNA-binding motifs and are thought to play an important role in sexual development.
DMRTB1 (Doublesex- and mab-3-related transcription factor B1) is a 342 amino acid protein that contains one DM DNA-binding domain and belongs to the DMRT family. Localized to the nucleus and expressed in the testis, DMRTB1 may participate in developmental processes and, via its DM domain, may bind to DNA and regulate transcription. DMRTB1 belongs to the DMRT family. Contains 1 DM DNA-binding domain.
