Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMRTA2 in samples. An antibody specific for DMRTA2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTA2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTA2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTA2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DMRTA2 is involved in gonadal differentiation. The gene is part of five novel human DM genes that map to three well-defined regions of chromosomes 1, 9, and 19. Several studies have reported QTL in various species and strains of tilapia, regions contributing to sex determination have been identified on linkage groups 1, 3, and 23. Genes contributing to sex-specific mortality have been detected on linkage groups 2, 6, and 23. Mapping of Dax1 joined LG16 and LG21 into a single linkage group. The Amh and Dmrta2 genes were mapped to two distinct regions of LG23. The Amh gene was mapped 5 cM from UNH879 within a QTL region for sex determination and 2 cM from UNH216 within a QTL region for sex-specific mortality. Dmrta2 was mapped 4 cM from UNH848 within another QTL region for sex determination.
