Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMRTA1 in samples. An antibody specific for DMRTA1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRTA1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRTA1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRTA1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DMRTA1, Belongs to the DMRT family. Contains 1 DM DNA-binding domain. Expressed in liver, kidney, pancreas, prostate and weakly detected in testis and ovary.May be involved in sexual development.
The Dmrt genes encode a large family of transcription factors whose function in sexual development has been well studied in invertebrates and vertebrates. Their expression pattern is not restricted to the developing gonads, indicating that Dmrt genes might regulate other developmental processes. By genomic analysis of zebrafish and rat DMRT genes, all protein sequences of the vertebrate DMRTs were searched from gene databases and aligned. Phylogenetic tree of all these DMRT genes was reconstructed and evaluated by Bootstrap method. These DMRT genes were clustered into seven subfamilies.
