• Product NameCCL8 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationAntigen Affinity Purified
• ApplicationsIHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total CCL8 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human C-C motif chemokine 8 protein(23-99aa)
• Target NameCCL8
• Alternative Names    HC14, Monocyte chemoattractant protein 2, Monocyte chemotactic protein 2, MCP-2, Small-inducible cytokine A8, CCL8, MCP2, SCYA10, SCYA8
• Accession No. Swiss-Prot#: P80075
• UniprotP80075
• Gene ID6355;
• Concentration 1.0mg/mL
• Formulation PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Storage Store at -20˚C

• 
  Immunohistochemistry: 1:20 - 1:200

Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. May play a role in neoplasia and inflammatory host responses. This protein can bind heparin. The processed form MCP-2(6-76) does not show monocyte chemotactic activity, but inhibits the chemotactic effect most predominantly of CCL7, and also of CCL2 and CCL5 and CCL8.

[1]Complete crystal structure of monocyte chemotactic protein-2, a CC chemokine that interacts with multiple receptors."Blaszczyk J., Coillie E.V., Proost P., Damme J.V., Opdenakker G., Bujacz G.D., Wang J.M., Ji X. Biochemistry 39:14075-14081(2000). [2]Posttranslational modifications affect the activity of the human monocyte chemotactic proteins MCP-1 and MCP-2: identification of MCP-2(6-76) as a natural chemokine inhibitor."Proost P., Struyf S., Couvreur M., Lenaerts J.-P., Conings R., Menten P., Verhaert P., Wuyts A., Van Damme J.J. Immunol. 160:4034-4041(1998). [3]Structural characterization of a monomeric chemokine: monocyte chemoattractant protein-3."Kim K.-S., Rajarathnam K., Clark-Lewis I., Sykes B.D.FEBS Lett. 395:277-282(1996).

• Immunohistochemical analysis of paraffin-embedded human small intestine using #42106 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting