Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMRT3 in samples. An antibody specific for DMRT3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMRT3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMRT3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMRT3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Vertebrate DM domain genes encode a novel group of proteins related to the Drosophila doublesex and Caenorhabditis elegans mab-3 transcription factors. It is shown here that the recently identified gene, DMRT3, has a restricted embryonic expression profile that is conserved in chicken and mouse embryos. DMRT3 is expressed primarily in the forebrain, neural tube and nasal placode of both species. In the chicken, DMRT3 is also expressed in newly forming tail somites at early developmental stages and, later, in the Mllerian ducts of the urogenital system.The sequence of this gene is defined by 14 GenBank accessions from 13 cDNA clones, some from colon, PCR rescued clones (seen 2 times), thalamus (2), colon (once), colon tumor RER+ (once), lung (once), parathyroid gland (once), parathyroid tumor (once) and 4 other tissues.
