Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DMPK in samples. An antibody specific for DMPK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDMPK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DMPK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DMPK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Myotonic dystrophy protein kinase is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.
