• Product NameHemoglobin Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityMs
• SpecificityThe antibody detects endogenous level of total Hemoglobin polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionMouse Hemoglobin protein
• Target NameHemoglobin
• Calculated MW 16kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Hemoglobin is involved in oxygen transport from the lung to the various peripheral tissues. The alpha (HBA) and beta (HBB) loci determine the structure of the 2 types of polypeptide chains in adult Hemoglobin. The normal adult Hemoglobin tetramer consists of two alpha chains and two beta chains. Mutant beta globin causes sickle cell anemia. Absence of beta chain causes beta zero thalassemia. Reduced amounts of detectable beta globin causes beta plus thalassemia.

• All lanes : Mouse Hemoglobin antibody at 2ug/ml+ Mouse serum Lane 1 : Mouse serum at 1:100 Lane 2 : Mouse serum at 1:1000 SecondaryGoat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 16 kDa Observed band size: 16kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting