Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DLG2 in samples. An antibody specific for DLG2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLG2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLG2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLG2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Palmitoylated membrane protein 2 is a member of a family of membrane-associated proteins termed MAGUKs (membrane-associated guanylate kinase homologs). MAGUKs interact with the cytoskeleton and regulate cell proliferation, signaling pathways, and intracellular junctions. Palmitoylated membrane protein 2 contains a conserved sequence, called the SH3 (src homology 3) motif, found in several other proteins that associate with the cytoskeleton and are suspected to play important roles in signal transduction.The dlg-A protein had been shown to act as a tumor suppressor, and the other members of this family may be involved in signal transduction through specialized membrane domains with highly organized cytoskeletons and thus are potential tumor suppressors.
