Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DLEU7 in samples. An antibody specific for DLEU7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDLEU7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DLEU7 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DLEU7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Deletion of chromosome 13q14 is the most frequent genetic aberration in B-cell chronic lymphocytic leukemia (CLL), found in more than 50% of cases, indicating that this region contains a gene(s) involved in the development of CLL.DLEU7, located adjacent to the consensus deleted region, and overlapping the 3' end of DLEU1 tail to tail. Human DLEU7 encodes a putative 221 amino acid protein, with significant conservation in rodents. Mutational and expression analysis in primary CLL samples failed to demonstrate any specific mutations in DLEU7, but no DLEU7 expression could be detected in CLL cells. Methylation of a CpG island in the promoter region of DLEU7 was further analyzed as a possible mechanism for the absence of DLEU7 expression, and the promoter was found to be methylated in the majority of the CLL samples investigated.
