Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DISC1 in samples. An antibody specific for DISC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDISC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DISC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DISC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Disrupted-in-Schizophrenia 1 or DISC1 is a protein with a wide array of functions. It participates in cell growth, movement, axonal growth and transport, neural positioning. Disruptions in DISC1 structure may play an important role at least in some cases of schizophrenia, however the evidence from genetic linkage and association studies suggests that variants of DISC1 do not play a significant role in most cases of schizophrenia, although DISC1 may be more important in bipolar disorder. In humans, this protein is encoded by the DISC1 gene.
This gene encodes a protein with multiple coiled coil motifs which is located in the nucleus, cytoplasm and mitochondria. The protein is involved in neurite outgrowth and cortical development through its interaction with other proteins.
