Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DIS3 in samples. An antibody specific for DIS3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIS3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIS3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIS3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:By randomly sequencing clones obtained from a size-fractionated brain cDNA library, Nagase et al. (1999) cloned DIS3, which they designated KIAA1008. The deduced 928-amino acid protein shares 44.5% identity with the mitotic control protein Dis3 of S. pombe. PCR-ELISA detected low to moderate expression in all tissues and brain regions tested, with highest expression in testis and moderate expression in kidney, ovary, corpus callosum, spinal cord, and fetal brain.
By sequence analysis of chromosome 13q21-q22 to identify a breast cancer susceptibility gene, Rozenblum et al. (2002) identified DIS3. The deduced protein contains 958 amino acids. Northern blot analysis detected ubiquitous expression.
