Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DIRC2 in samples. An antibody specific for DIRC2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIRC2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIRC2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIRC2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:DIRC2 has a conserved motif that is shared with the major facilitator superfamily of transporters between membrane-spanning domains 2 and 3, and a proline-rich region between membrane-spanning domains 6 and 7. DIRC2 also contains a putative N-glycosylation site and several putative phosphorylation sites. Computer predictions of the putative DIRC2 protein showed significant homology to different members of the major facilitator superfamily of transporters. DIRC2 shares 43% similarity with the human homolog of feline leukemia virus type C receptor (FLVXR), which has been classified as a major facilitator superfamily transporter, and more than 85% homology with Dirc2 from monkey, pig, dog, and mouse. Northern blot analysis revealed a 2.2-kb transcript in most tissues tested, including kidney.
