Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate DIRC1 in samples. An antibody specific for DIRC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyDIRC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DIRC1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DIRC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Podolski et al. (2001) described a reciprocal, balanced, constitutional chromosome translocation, t(2;3)(q33;q21), that is associated with familial clear cell renal cancer. The gene, designated DIRC1 (disrupted in renal cancer-1), was disrupted between exons 1 and 2 by the familial translocation. The 1.5-kb DIRC1 mRNA encoded an 11-kD predicted protein of 104 amino acids. RT-PCR analysis detected low-level expression of DIRC1 in adult placenta, testis, ovary, and prostate, and in fetal kidney, spleen, and skeletal muscle. Two familial tumors showed loss of the derivative chromosome 3, as observed in a Dutch kindred with t(2;3)-associated renal cancers in a Dutch family. Druck et al. (2001) concluded that further studies were necessary to determine if inactivation of the gene contributes to the development of familial cancers.
