• Product NameClathrin heavy chain 2 Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Clathrin heavy chain 2 polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Clathrin heavy chain 2 protein
• Target NameClathrin heavy chain 2
• Alternative Names    Clathrin heavy chain on chromosome 22, CLH-22, CLTCL1, CLH22, CLTCL, CLTD
• Accession No. Swiss-Prot#: P53675
• UniprotP53675
• Gene ID8218;
• Calculated MW 187kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network

[1]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.S

• All lanes:Clathrin heavy chain 2 antibody at 2ug/ml Lane 1:Jurkat whole cell lysate Lane 2:NIH3T3 whole cell lysate Lane 3:A431 whole cell lysate secondary Goat polyclonal to rabbit at 1/10000 dilution predicted band size :187kDa observed band size : 187kDa

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting