• Product NameThiopurine S-methyltransferase Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Thiopurine S-methyltransferase polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Thiopurine S-methyltransferase protein
• Target NameThiopurine S-methyltransferase
• Alternative Names    Thiopurine methyltransferase
• Accession No. Swiss-Prot#: P51580
• UniprotP51580
• Gene ID7172;
• Calculated MW 27kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

TPMT is an enzyme that metabolizes thiopurine drugs via S-adenosyl-L-methionine as the S-methyl donor and S-adenosyl-L-homocysteine as a byproduct. Thiopurine drugs such as 6-mercaptopurine are used as chemotherapeutic agents. Genetic polymorphisms that affect this enzymatic activity are correlated with variations in sensitivity and toxicity to such drugs within individuals. A pseudogene for this locus is located on chromosome 18q.

[1] "Human thiopurine methyltransferase: molecular cloning and expression of T84 colon carcinoma cell cDNA."Honchel R., Aksoy I.A., Szumlanski C., Wood T.C., Otterness D.M., Wieben E.D., Weinshilboum R.M.Mol. Pharmacol. 43:878-887(1993) [2]

• All lanes : Thiopurine S-methyltransferase antibody at 2ug/ml Lane 1 : EC109 whole cell lysate Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 27kDa Observed band size: 27kDa
• Immunohistochemical analysis of paraffin-embedded human kidney using #42134 at dilution of 1:100.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting