• Product NameTransgelin Polyclonal Antibody
• Host SpeciesRabbit
• ClonalityPolyclonal
• PurificationCaprylic Acid Ammonium Sulfate Precipitation purified
• ApplicationsWB IHC
• Species ReactivityHu
• SpecificityThe antibody detects endogenous level of total Transgelin polyclonal antibody.
• Immunogen Typeprotein
• Immunogen DescriptionRecombinant human Transgelin protein
• Target NameTransgelin
• Alternative Names    22 kDa actin-binding protein, Protein WS3-10, Smooth muscle protein 22-alpha, SM22-alpha
• Accession No. Swiss-Prot#: Q01995
• UniprotQ01995
• Gene ID6876;
• Calculated MW 22kd
• Formulation Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
• Storage Store at -20˚C

• Western blotting: 1:500 - 1:1000
  Immunohistochemistry: 1:20 - 1:200

Actin cross-linking/gelling protein By similarity. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence.

• All lanes : Transgelin antibody at 2ug/ml Lane 1 : EC109 whole cell lysate Lane 2 : 293T whole cell lysate Secondary Goat polyclonal to Rabbit IgG at 1/15000 dilution Predicted band size : 22 kDa Observed band size: 22 kDa
• Immunohistochemical analysis of paraffin-embeded human umbilical cord using #42165 at dilution of 1:50.

• Flow Cytometry (FCM) General Protocol
• Immunofluorescence(IC)
• Immunohistochemistry(Polymer)
• Western Blotting