Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate Cr in samples. An antibody specific for Cr has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCr present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cr is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cr bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Creatinine is a breakdown product of creatine phosphate in muscle, and is usually produced at a fairly constant rate by the body. Serum creatinine is an important indicator of renal health because it is an easily measured byproduct of muscle metabolism that is excreted unchanged by the kidneys. Creatinine itself is produced via a biological system involving creatine, phosphocreatine, and adenosine triphosphate. Creatine is synthesized primarily in the liver from the methylation of glycocyamine (guanidino acetate, synthesized in the kidney from the amino acids arginine and glycine) by S-adenosyl methionine. It is then transported through blood to the other organs, muscle, and brain, where, through phosphorylation, it becomes the high-energy compound phosphocreatine. During the reaction, creatine and phosphocreatine are catalyzed by creatine kinase, and a spontaneous conversion to creatinine may occur.
