Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate NOS1 in samples. An antibody specific for NOS1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNOS1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NOS1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NOS1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Nitric oxide (NO) is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter; it is implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. NO is also responsible for endothelium-derived relaxing factor activity regulating blood pressure. NO mediates tumoricidal and bactericidal actions, as indicated by the fact that inhibitors of NO synthase (NOS) block these effects. Neuronal NOS and macrophage NOS are distinct isoforms.Both the neuronal and the macrophage forms are unusual among oxidative enzymes in requiring several electron donors: FAD, flavin mononucleotide (FMN), NADPH, and tetrahydrobiopterin.
