Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate NEXN in samples. An antibody specific for NEXN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNEXN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NEXN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NEXN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The name nexilin came from the Latin word nexilis, meaning 'bound together.' Full-length b-nexilin contains an N-terminal F-actin-binding domain (ABD), followed by a spacer, a coiled-coil region, a second ABD, and a long C-terminal tail. In comparison, s-nexilin lacks the N-terminal ABD and has an insertion in the C-terminal tail. Immunofluorescence microscopy revealed that both b-nexilin and s-nexilin colocalized with F-actin at focal contacts at the ends of stress fibers.
The nexilins did not localize to cell-cell junctions. Western blot analysis detected nexilin proteins with apparent molecular masses above 90 kD in rat brain, testis, and spleen and in cultured rodent fibroblasts. Neither protein was detected in liver, kidney, or epithelial cell lines.
