Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate NADK in samples. An antibody specific for NADK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyNADK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NADK is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NADK bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:kinase (EC 2.7.1.23, NADK) is an enzyme that converts nicotinamide adenine dinucleotide (NAD+) into NADP+, through phosphorylating the NAD+ coenzyme. NADP+ is an essential coenzyme in metabolism and provides reducing power to biosynthetic processes such as fatty acid biosynthesis. The structure of the NADK from the archaean Archaeoglobus fulgidus has been determined.
The deduced 446-amino acid protein has a calculated molecular mass of 49.2 kD. Northern blot analysis detected a 3.0-kb transcript with high expression in placenta, moderate expression in brain, colon, heart, liver, kidney, lung, spleen, stomach, and testis, and low expression in small intestine. A band of 1.8 kb was detected in several tissues.
