Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate MMP-4 in samples. An antibody specific for MMP-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMMP-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MMP-4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MMP-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases.
The protein encoded by this gene is considered a member of the membrane-type MMP (MT-MMP) subfamily. However, this protein is unique among the MT-MMP's in that it is a GPI-anchored protein rather than a transmembrane protein. The protein activates MMP-2 by cleavage.In melanocytic cells MMP17 gene expression may be regulated by MITF.
