Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate KT in samples. An antibody specific for KT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyKT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Transketolase (EC 2.2.1.1) is a thiamine-dependent enzyme that links the pentose phosphate pathway with the glycolytic pathway. The pentose phosphate pathway, which is active in most tissues, provides sugar phosphates for intermediary biosynthesis, especially nucleotide metabolism, and generates the biosynthetic reducing power for the cell in the form of NADPH. Transketolase is directly involved in the branch of the pathway that channels excess sugar phosphates to glycolysis, enabling the production of NADPH to be maintained under different metabolic conditions. NADPH is critical for maintaining cerebral glutathione, and thus it is likely that transketolase plays an important role in brain metabolism.
