Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate IP3 in samples. An antibody specific for IP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IP3 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Inositol trisphosphate is a secondary messenger molecule used in signal transduction in biological cells. It is made by hydrolysis of phosphatidylinositol 4,5-bisphosphate (PIP2), a phospholipid that is located in the plasma membrane, by phospholipase C. IP3 binds to and activates the InsP3 receptor on the membrane of the endoplasmic reticulum (ER), and sarcoplasmic reticulum (SR) opens a calcium channel, resulting in the release of Ca2 into the cytoplasm, and sarcoplasm respectively. This increase in Ca2 activates the ryanodine receptor-operated channel on the SR, leading to a further increase in the Ca2 .
Its main functions are to mobilize Ca2+ from storage organelles and to regulate cell proliferation and other cellular reactions.In smooth muscle cells, for example, the increase in concentration of cytoplasmic calcium results in the contraction of the muscle cell.
