Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate IHH in samples. An antibody specific for IHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IHH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IHH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:IHH is one of three proteins in the mammalian hedgehog family, the others being desert hedgehog (DHH) and Sonic hedgehog (SHH). It is involved in chondrocyte differentiation, proliferation and maturation especially during endochondral ossification. It regulates its effects by feedback control of parathyroid hormone-related peptide (PTHrP).Gao et al. (2001) decided to focus on IHH as the gene implicated in brachydactyly type A1 because of its position in the implicated interval on chromosome 2q35-q36 flanked by markers D2S2248 and D2S360 and becauseIHH is known to mediate condensation, growth, and differentiation of cartilage. They identified 3 heterozygous missense mutations in the region encoding the amino-terminal signaling domain in all affected members of 3 large, unrelated Chinese families.
