Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate IFNA21 in samples. An antibody specific for IFNA21 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyIFNA21 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFNA21 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFNA21 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:The IFNA21 gene was positioned in the cluster of interferon genes on 9p22 by deletion mapping. Interferons (IFNs) are proteins made and released by lymphocytes in response to the presence of pathogenssuch as viruses, bacteria, or parasitesor tumor cells. IFNs belong to the large class of glycoproteins known as cytokines. Although they are named after their ability to "interfere" with viral replication within host cells, IFNs have other functions: they activate immune cells, such as natural killer cells and macrophages; they increase recognition of infection or tumor cells by up-regulating antigen presentation to T lymphocytes; and they increase the ability of uninfected host cells to resist new infection by virus. Certain host symptoms, such as aching muscles and fever, are related to the production of IFNs during infection.
