Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate HSF2 in samples. An antibody specific for HSF2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyHSF2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSF2 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSF2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.Kallio et al. (2002) found that mouse Hsf2 was expressed in all 3 embryonic layers at embryonic day 7.5 and that the head fold was strongly stained at embryonic day 8.5. At later developmental stages, Hsf2 expression was progressively limited to the central nervous system. In adults, Hsf2 expression was detected in spermatocytes and spermatogonia, but not in elongated spermatids, spermatozoa, or Sertoli cells.
