Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate GAST in samples. An antibody specific for GAST has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyGAST present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GAST is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GAST bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Gastrin is a major physiological regulator of gastric acid secretion. It also has an important trophic or growth-promoting influence on the gastric mucosa. Gastrin is synthesized in G cells, which are located in gastric pits, primarily in the antrum region of the stomach and binds receptors found predominantly on parietal and enterochromaffin-like cells.In humans, gastrin is a hormone that stimulates secretion of gastric acid by the parietal cells of the stomach.
It is released by G cells in the stomach and duodenum.Gastrin is a linear peptide hormone produced by G cells of the duodenum and in the pyloric antrum of the stomach. In the Zollinger-Ellison syndrome, gastrin is produced at excessive levels, often by a gastrinoma of the antrum or the pancreas.
