Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate FSIP1 in samples. An antibody specific for FSIP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyFSIP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FSIP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FSIP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:FSIP1, also known as HDS10, is a recently discovered gene that encodes fibrous sheath interacting protein 1, and is regulated by amyloid beta precursor protein (APP). Recently, it has been reported that a peptide derived from APP is cleaved by alpha disintegrin and metalloproteinase 33 (ADAM33), which is an asthma susceptibility gene. It has also been known that the FSIP1 gene is expressed in airway epithelium. FSIP1 gene might be a susceptibility gene for aspirin intolerance in asthmatics. AKAP4 binds AKAP3 and two novel spermatogenic cell-specific proteins,Fibrous Sheath Interacting Proteins 1 and 2 (FSIP1, FSIP2). Transcription of Akap4, Akap3, and Fsip1 begins in early spermatid development, whereas transcription of Fsip2 begins in late spermatocyte development.
