Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CRNN in samples. An antibody specific for CRNN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCRNN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRNN is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRNN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Cornulin is a member of the 'fused gene' family of proteins, which contain N-terminus EF-hand domains and multiple tandem peptide repeats. The encoded protein contains two EF-hand Ca2+ binding domains in its N-terminus and two glutamine- and threonine-rich 60 amino acid repeats in its C-terminus. This gene, also known as squamous epithelial heat shock protein 53, may play a role in the mucosal/epithelial immune response and epidermal differentiation.
The C1ORF10 cDNA encodes a deduced 490-amino acid protein with 1 transmembrane domain, an N-terminal EF-hand calcium-binding motif of about 90 amino acids that shares 25 to 35% identity to human S100 calcium-binding proteins, and a conserved consecutive repeat sequence of about 60 amino acids.
