Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CRH in samples. An antibody specific for CRH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCRH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRH is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:releasing factor was initially isolated from ovine hypothalamus by Vale et al., in 1981, and identified as a novel neuropeptide comprising 41 amino acid residues with molecular weight 4758. Later human CRF and rat CRF were also isolated and identified. The mouse CRF peptide is identical at amino acid level to the rat and human CRF peptides. CRF in anterior pituitary promotes the synthesis and secretion of ACTH, a main factor of hypothalamus-pituitary-adrenal (HPA) axis. In the rat and human, CRF distributes mainly in hypothalamus, but it was also found in spinal cord, stomach, spleen, duodenum, adrenal and placenta. In addition, immunochemical evidence supported the wide distribution of the peptide throughout the central nervous system (CNS) such as olfactory bulb, retina and central auditory system in the rat.
