Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CORT in samples. An antibody specific for CORT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCORT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CORT is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CORT bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Corticosterone is a glucocorticoid produced by the adrenal cortex in response to ACTH (adrenocoridotropic hormone), and is the precursor to aldosterone. The production of glucorcorticoids is increased by stress; therefore, corticosterone can be used as a biomarker of stress. Biologists often measure corticosterone in fecal material of the species they are studying.
This non-invasive sample collection procedure has the advantage of not itself causing stress, and thereby increasing corticosterone levels, a common problem where samples for measurement of corticosterone are collected by more invasive means. Plasma corticosterone levels have a circadian variation, and may be important in the regulation of the sleep-wake cycle.Corticosterone (CORT) is a 21 carbon steroid hormone of the corticosteroid type produced in the cortex of the adrenal glands.
