Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CCL28 in samples. An antibody specific for CCL28 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCCL28 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CCL28 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CCL28 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:CCL28 is a chemokine. CCL28 regulates the chemotaxis of cells that express the chemokine receptors CCR3 and CCR10. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3.
This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury. CCL28 has also been implicated in the migration of IgA-expressing cells to the mammary gland, salivary gland, intestine and other mucosal tissues.
