Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CALU in samples. An antibody specific for CALU has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCALU present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CALU is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CALU bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Calumenin (CALU) is a calcium-binding protein localized in the endoplasmic reticulum (ER) and is involved in such ER functions as protein folding and sorting. Calumenin is a member of the EF-hand superfamily in the ER and Golgi apparatus named CERC. CERC is an acronym for its family members Cab-45, reticulocalbin, Erc-55, and calumenin.
The CALU gene encodes a deduced 315-amino acid protein containing 6 EF-hand motifs, 1 potential N-glycosylation site, and a C-terminal ER retention signal. The human and mouse CALU proteins are 98% identical. CALU mRNA is ubiquitously expressed in human tissues and maps to 7q32.
