Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate CACNA1C in samples. An antibody specific for CACNA1C has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyCACNA1C present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CACNA1C is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CACNA1C bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Activation of voltage-sensitive calcium channels by membrane depolarization triggers key cellular responses such as contraction, secretion, excitation, and electrical signaling. The L-type currents produced by voltage-sensitive calcium channels are blocked by 1,4-dihydropyridine (DHP) derivatives; thus, the channels responsible for these currents are referred to as DHP-sensitive. The skeletal muscle DHP-sensitive calcium channel is a complex of 5 subunits: alpha-1, alpha-2, beta, gamma, and delta. The DHP-sensitive calcium channels from cardiac muscle and the brain have pharmacologic and electrophysiologic properties that differ from those of the skeletal muscle channel. Powers et al. (1991) isolated a clone for the human CCHL1A1 gene and partially sequenced it.
