Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate BECN1 in samples. An antibody specific for BECN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBECN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BECN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BECN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Beclin-1 is also known as autophagy-related gene (Atg) 6. Beclin-1 and its binding partner class III phosphoinositide 3-kinase (PI3K), also named Vps34, are required for the initiation of the formation of the autophagasome in autophagy.Human beclin encodes a novel 450-amino acid protein containing a coiled-coil region, within which it has limited homology to myosin-like proteins . Human and mouse beclin share 98% amino acid sequence identity. Caenorhabditis elegans and Saccharomyces cerevisiae beclin homologs were also identified. PROSITE analysis of human beclin detected several potential glycosylation, phosphorylation, and myristoylation sites, but no other functional sequence motifs. Northern blot analysis of mouse and adult human tissues revealed widespread beclin expression.
