Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate BCL2A1 in samples. An antibody specific for BCL2A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyBCL2A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BCL2A1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BCL2A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:BCL2A1encodes a member of the bcl2 protein family. The proteins of this family form hetero- or homodimers and act as anti- and pro-apoptotic regulators that are involved in a wide variety of cellular activities such as embryonic development, homeostasis and tumorigenesis. The protein encoded by this gene is able to reduce the release of pro-apoptotic cytochrome c from mitochondria and block caspase activation. This gene is a direct transcription target of NF-kappa B in response to inflammatory mediators, and has been shown to be up-regulated by different extracellular signals, such as granulocyte-macrophage colony-stimulating factor (GM-CSF), CD40, phorbol ester and inflammatory cytokine TNF and IL-1, which suggests a cytoprotective function that is essential for lymphocyte activation as well as cell survival.
