Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate TMSB4Y in samples. An antibody specific for TMSB4Y has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMSB4Y present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMSB4Y is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMSB4Y bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:Lahn and Page (1997) isolated testis cDNAs encoding TB4Y, a Y-linked TB4X homolog. They identified TB4Y as 1 of 5 NRY genes that were expressed in many tissues and have homologs on the X chromosome that escape X inactivation.
The antigen, an 11-mer peptide (EVLLRPGLHFR), was encoded by a polymorphic region 405 bp upstream of the initiation codon of TMSB4Y. CTL precursor frequency analysis showed that approximately 25% of the total donor CTL responses in the patient 50 days after transplant were directed at this epitope, and the precursor was detectable almost 2 years after transplant. RT-PCR analysis detected wide expression of TMSB4Y mRNA in normal tissues, including hematopoietic cells, and TMSB4Y was also expressed in some leukemic cells.
